75. Gestational Exposures to Phthalates and Folic Acid, and Autistic Traits in Canadian Children (abstract)

Oulhote Y, Lanphear BP, Braun JM, Webster GM, Arbuckle TE, Etzel T, Forget-Dubois N, Séguin JR, Bouchard MF, MacFarlane AJ, Ouellet E, Fraser WD, Muckle G. Environmental Health Perspectives 2020 Feb;128(2):27004. doi: 10.1289/EHP5621

Background: The etiology of autism spectrum disorder is poorly understood. Few studies have investigated the link between endocrine-disrupting chemicals and autistic traits. We examined the relationship between gestational phthalates and autistic traits in 3- to 4-y-old Canadian children. We also investigated potential effect modification by sex and folic acid supplementation.

Methods: We enrolled 2,001 of age during the first trimester of pregnancy between 2008 and 2011 from 10 cities in Canada. At 3-4 years of age, 610 children underwent neuropsychological assessments including the Social Responsiveness Scale-II (SRS-2) as a measure of autistic traits and social impairment. We measured 11 phthalate metabolites in maternal first trimester urine samples and assessed folic acid supplementation from reported intakes. We estimated covariate-adjusted differences in SRS-2 T-scores with a doubling in phthalate concentrations in 510 children with complete data.

Results: Mean total SRS T-score was 45.3 (). Children with higher gestational exposure to mono-n-butyl (MBP) and mono-3-carboxypropyl (MCPP) concentrations exhibited significantly higher total SRS T-scores, indicating greater overall social impairment, as well as higher scores on subdomains, indicating deficits in social cognition, social communication, social motivation, and restricted interests/repetitive behaviors. A doubling in MBP or MCPP concentrations was associated with 0.6 (95% CI: 0.1, 1.0) and 0.5 (95% CI: 0.1, 0.8) higher total SRS T-scores. Associations were consistently and significantly stronger in boys (= 1.0; 95% CI: 0.4, 1.6; ) compared with girls (; 95% CI: 0.7; ) and among children who had lower prenatal folic acid supplementation () (; 95% CI: 0.4, 2.3; ) compared with those who had adequate folic acid supplementation () (; 95% CI: , 0.8; ).

Conclusions: Higher gestational concentrations of some phthalate metabolites were associated with higher scores of autistic traits as measured by the SRS-2 in boys, but not girls; these small size effects were mitigated by first trimester-of-pregnancy folic acid supplementation. https://doi.org/10.1289/EHP5621.